phosphorylated acc1 (ser79  (Cell Signaling Technology Inc)

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Effect of His Bundle Pacing on Abnormal Myocardial Fatty Acid and Glucose Metabolism Induced by Right Ventricular Pacing

doi: 10.1161/JAHA.123.032386

Figure Lengend Snippet: In terms of regulatory proteins related to fatty acid metabolism, we checked the key proteins, such as CD36, CPT1, ACC1 and HSL. A , The expression of CD36 was higher in the RV pacing group than the sham control and His pacing groups. The expression of CPT1 ( B ) and p‐ACC1/ACC1 ( C ) did not differ among the 3 groups. D , The expression of HSL was significantly lower in the RV pacing group compared with the sham control and His pacing groups. E , We also evaluated the inhibitory form of HSL ( p565 HSL), and the expression of p565 HSL/HSL was significantly lower in the RV pacing group compared with the sham control and His pacing groups. * P <0.05; ** P <0.01. ACC1 indicates acetyl‐CoA carboxylase 1; CPT1, carnitine palmitoyltransferase I; HSL, hormone‐sensitive lipase; and p‐ACC1, phosphorylated acetyl‐CoA carboxylase 1.

Article Snippet: The primary antibodies against binding immunoglobulin protein (Bip), glucose transporter 4 (GLUT4), hormone‐sensitive lipase (HSL), pyruvate dehydrogenase (PDH), acetyl‐CoA carboxylase 1 (ACC1), phosphorylated ACC1 (Ser79), phosphorylated HSL (Ser565) (1:1000 dilution; Cell Signaling Technology, Danvers, MA), tumor necrosis factor‐α (TNFα; 1:200 dilution; Santa Cruz, TX), and carnitine palmitoyltransferase I (CPT1) (1:5000 dilution; Abcam, Cambridge, MA) were used to react with the blots at 4 °C overnight in 5% nonfat dry milk.

Techniques: Expressing

Journal: Journal of Alzheimer's disease : JAD

Article Title: OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer’s Disease Cytoplasmic Hybrids

doi: 10.3233/JAD-200996

Figure Lengend Snippet: AD cybrids show decreased AMPK expression. A) AMPK and mTOR nutrient sensing pathway levels in SH-SY5Y whole-cell lysates. Box plots represent quantitation of mTOR (B), phosphorylated ACC1 (C), ACC1 (D), AMPK (E), phosphorylated 4EBP1 (F), and 4EBP1 (G) bands with citrate synthase (CS) used as a loading control. The colored region is the inter-quartile range while the bars represent points falling within 1.5 times the IQR. *p < 0.05. **p < 0.01. ***p < 0.001. WB, western blotting; Con, control; AD, Alzheimer’s disease; TMG, Thiamet G.

Article Snippet: The following antibodies were purchased from Cell Signaling Technologies: AMPK (5831), ACC1 (4190), phosphorylated-ACC1 (11818), mTOR (2972), 4EBP1 (9644), phosphorylated-4EBP1 (2855), and Sirtuin 3 (C73E3).

Techniques: Expressing, Quantitation Assay, Control, Western Blot

Journal: The Journal of nutrition

Article Title: Cholic Acid Supplementation of a High-Fat Obesogenic Diet Suppresses Hepatic Triacylglycerol Accumulation in Mice via a Fibroblast Growth Factor 21-Dependent Mechanism.

doi: 10.1093/jn/nxy022

Figure Lengend Snippet: FIGURE 4 Abundance of P-ACC1 (Ser79) and total ACC1 in liver ex- tracts (A) and the abundance of P-HSL (Ser660 and Ser565) and total HSL in adipose extracts (B) of WT mice and FGF21KO mice fed an STD or an HFD for 23 wk or an HFD for 19 wk followed by 4 wk of HFD+CA. The ratio of P-ACC1 to total ACC1 and the ratio of P-HSL to total HSL in extracts prepared from WT mice fed the STD were set at 1 and the other values were adjusted proportionately. Values are means ± SEMs, n = 8. Labeled means without a common let- ter differ, P < 0.05. ACC1, acetyl-CoA carboxylase 1; CA, cholic acid; FGF21KO, fibroblast growth factor 21 knockout; HFD, high-fat obeso- genic diet; HSL, hormone-sensitive lipase; P-, phosphorylated; STD, standard diet; WT, wild-type.

Article Snippet: Antibodies against phosphorylated acetyl-CoA carboxylase 1 (ACC1) Ser79, phosphorylated hormone sensitive lipase (HSL) Ser565 and Ser660, total ACC1, and total HSL were obtained from Cell Signaling Technology.

Techniques: Labeling, Knock-Out

Journal: Physiological Reports

Article Title: Skeletal muscle IL ‐6 and regulation of liver metabolism during high‐fat diet and exercise training

doi: 10.14814/phy2.12788

Figure Lengend Snippet: Hepatic ACC 1 Ser79 phosphorylation normalized to ACC 1 protein content (A), ACC 1 protein content (B), FAS protein content (C), ACC 2 Ser212 phosphorylation normalized to ACC 2 protein content (D), ACC2 protein content (E) and representative blots (F) in Floxed and IL ‐6 MKO mice after 16 weeks on Chow, HFD or HFD with exercise training ( n = 9–10). Values are mean ± SE . *Significantly different from Chow within given genotype ( P < 0.05).

Article Snippet: The membrane was blocked in 3% fish gel (FG) and probed over night with primary antibodies recognizing ACC1 Ser79 and 2 Ser212 phosphorylation (#07‐303, Millipore, Darmstadt, Germany), ACC1 and 2 (Streptavidin‐HRP, Dako, Denmark), respiratory chain complexes I‐V (#110413, ABCAM, Cambridge, UK), PEPCK (#10004943, Cayman chemicals, Ann Abor, MI), PDK2 (# ST1643, Calbiochem, Millipore), PDK4 (kind gift from professor Grahame Hardie, University of Dundee, UK), G6Pase (#27198, Santa Cruz biotechnology, Dallas, TX) and GAPDH (# 2118, Cell Signaling Technologies, Beverly, MA).

Techniques: Phospho-proteomics